1SAT

CRYSTAL STRUCTURE OF THE 50 KDA METALLO PROTEASE FROM S. MARCESCENS

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	151.000, 109.200, 42.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.750
R-factor	0.188
Rwork	0.188
RMSD bond length	0.009
RMSD bond angle	26.460 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.700 *	1.700 *
Rmerge	0.075 *
Total number of observations	253857 *
Number of reflections	81141
Completeness [%]	95.0	82 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	30 (%)
2	1	reservoir	ammonium sulfate	0.1 (M)
3	1	reservoir	citrate	0.1 (M)