1SA9

Crystal Structure of the RNA octamer GGCGAGCC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1995-04-03
Detector	RIGAKU RAXIS II
Wavelength(s)	1.5418
Spacegroup name	P 64
Unit cell lengths	69.650, 69.650, 68.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	60.320 - 2.860
Rwork	0.241
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.074
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.320	2.900
High resolution limit [Å]	2.800	2.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	sodium cacodylate, PEG8000, Zn(acetate)2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	sodium cacodylate
2	1	1	PEG8000
3	1	1	Zn(acetate)2
4	1	2	sodium cacodylate
5	1	2	PEG8000
6	1	2	Zn(acetate)2