1S9F
DPO with AT matched
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2002-05-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9160 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 104.800, 100.300, 110.800 |
| Unit cell angles | 90.00, 95.20, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.213 |
| Rwork | 0.205 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jx4 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.060 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.060 | 0.326 |
| Number of reflections | 148618 | |
| <I/σ(I)> | 20.2 | 3.2 |
| Completeness [%] | 95.5 | 68.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG 3350, ADA, ammonium acetate, calcium acetate, glycerol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ammonium acetate | ||
| 2 | 1 | 1 | calcium acetate | ||
| 3 | 1 | 1 | glycerol | ||
| 4 | 1 | 1 | PEG 3350 | ||
| 5 | 1 | 1 | ADA | ||
| 6 | 1 | 2 | PEG 3350 | ||
| 7 | 1 | 2 | ADA | ||
| 8 | 1 | 2 | calcium acetate |
