1S99
The structure and function of B. subtilis YkoF gene product: ligand free protein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2003-08-29 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9642, 0.9795, 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.930, 83.280, 85.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.300 - 1.650 |
| R-factor | 0.17353 |
| Rwork | 0.172 |
| R-free | 0.22375 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.517 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.322 | |
| Number of reflections | 51209 | |
| <I/σ(I)> | 24 | 1.9 |
| Completeness [%] | 43.4 | |
| Redundancy | 4.1 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 20% PEG 8000, 0.2 M calcium acetate, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295.0K |
