1S98
E.coli IscA crystal structure to 2.3 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2002-12-20 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 66.252, 66.252, 150.850 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.920 - 2.300 |
R-factor | 0.215 |
Rwork | 0.212 |
R-free | 0.24967 |
Structure solution method | SAD |
RMSD bond length | 0.038 |
RMSD bond angle | 2.507 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.350 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 20165 | |
<I/σ(I)> | 7.2 | 2 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 8.2 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 296 | NaPhosphate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 23K |