1S96
The 2.0 A X-ray structure of Guanylate Kinase from E.coli
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2002-08-22 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97934 |
Spacegroup name | P 21 3 |
Unit cell lengths | 124.552, 124.552, 124.552 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.550 - 2.000 |
Rwork | 0.195 |
R-free | 0.24700 |
Structure solution method | SAD |
RMSD bond length | 0.024 |
RMSD bond angle | 2.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.550 | 2.130 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.086 | |
Number of reflections | 46380 | |
<I/σ(I)> | 9 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 20 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 278 | PEG 400, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 278K |