1S8J

Nitrate-bound D85S mutant of bacteriorhodopsin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-09
Detector	ADSC QUANTUM 210
Wavelength(s)	1.1272
Spacegroup name	C 2 2 21
Unit cell lengths	52.950, 121.080, 74.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	11.980 - 2.300
R-factor	0.219
Rwork	0.219
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jv7
RMSD bond length	0.008
RMSD bond angle	1.000
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.440
High resolution limit [Å]	2.300	2.300
Number of reflections	11603
<I/σ(I)>	7.6
Completeness [%]	99.0	96.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1