1S89

H98N Mutant of Methylglyoxal Synthase from E. coli complexed with Phosphoglycolic Acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	2002-05-09
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	53.479, 129.497, 178.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.240 - 2.220
Rwork	0.190
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.240	2.240
High resolution limit [Å]	2.220	2.200
Number of reflections	60966

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	298	PEG 1500, Sodium Cacodylate, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K