1S7Y
Crystal structure of the GluR6 ligand binding core in complex with glutamate at 1.75 A resolution orthorhombic form
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9B |
Synchrotron site | NSLS |
Beamline | X9B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-21 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.96746, 0.97892, 0.97930 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.101, 91.187, 105.447 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.510 - 1.750 |
R-factor | 0.22 |
Rwork | 0.220 |
R-free | 0.24300 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.047 | 0.177 |
Number of reflections | 56491 | |
<I/σ(I)> | 20.6 | 6.12 |
Completeness [%] | 99.1 | 98.5 |
Redundancy | 3.3 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | 14% PEG 3350, 10 Citrate, 20 NaCl, 1 EDTA, 10 NaGlutamate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |