1S7X

Crystal structures of the murine class I major histocompatibility complex H-2Db in complex with LCMV-derived gp33 index peptide and three of its escape variants

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	CCD
Collection date	2003-03-12
Detector	MARRESEARCH
Wavelength(s)	1.007
Spacegroup name	P 1 21 1
Unit cell lengths	91.897, 123.318, 100.201
Unit cell angles	90.00, 102.82, 90.00

Refinement procedure

Resolution	19.800 - 2.410
Rwork	0.203
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1n5a
RMSD bond length	0.012
RMSD bond angle	1.250
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.800	2.540
High resolution limit [Å]	2.410	2.410
Number of reflections	83027
Completeness [%]	98.2	89.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Ammonium sulfate, Tris , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K