1S7V
Crystal structures of the murine class I major histocompatibility complex H-2Db in complex with LCMV-derived gp33 index peptide and three of its escape variants
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Detector technology | CCD |
Collection date | 2003-07-26 |
Detector | MARRESEARCH |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 160.469, 91.184, 92.147 |
Unit cell angles | 90.00, 125.00, 90.00 |
Refinement procedure
Resolution | 32.440 - 2.200 |
R-factor | 0.22761 |
Rwork | 0.226 |
R-free | 0.26678 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1n5a |
RMSD bond length | 0.016 |
RMSD bond angle | 1.401 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.000 | 2.257 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 52163 | |
Completeness [%] | 94.4 | 94.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Ammonium sulfate, Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |