1S7S
Crystal structures of the murine class I major histocompatibility complex H-2Kb in complex with LCMV-derived gp33 index peptide and three of its escape variants
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.069 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 88.262, 135.555, 45.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.880 - 1.990 |
| R-factor | 0.19206 |
| Rwork | 0.190 |
| R-free | 0.23191 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n59 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.694 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Number of reflections | 36362 | |
| Completeness [%] | 92.9 | 80.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | sodium/potassium phosphate, Methyl pentane diol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
