1S7R
Crystal structures of the murine class I major histocompatibility complex H-2Kb in complex with LCMV-derived gp33 index peptide and three of its escape variants
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-01-29 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.097 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.192, 88.519, 120.026 |
| Unit cell angles | 90.00, 94.00, 90.00 |
Refinement procedure
| Resolution | 24.840 - 2.950 |
| R-factor | 0.24165 |
| Rwork | 0.239 |
| R-free | 0.28961 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n59 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.493 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.100 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Number of reflections | 20741 | |
| Completeness [%] | 93.6 | 89.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | sodium/potassium phosphate, Methyl pentane diol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
