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1S7L

RimL- Ribosomal L7/L12 alpha-N-protein acetyltransferase in complex with Coenzyme A (CoA-Cys134 Disulfide)

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU RU200
Temperature [K]77
Detector technologyIMAGE PLATE
Collection date2003-03-03
DetectorRIGAKU RAXIS IV
Wavelength(s)1.5418
Spacegroup nameP 41 21 2
Unit cell lengths92.920, 92.920, 55.670
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution30.000 - 2.300
R-factor0.193
Rwork0.193
R-free0.24500
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1s7f
RMSD bond length0.022
RMSD bond angle2.000
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareAMoRE
Refinement softwareCNS (1.0)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.0002.380
High resolution limit [Å]2.3002.300
Number of reflections10924
<I/σ(I)>20.8
Completeness [%]96.693.7
Redundancy53.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1vapor diffusion under oil8293pentaerythritiol ethoxylate 797, triethanolamine, ammonium sulfate, acetylcoenzyme A, DTT, pH 8, vapor diffusion under oil, temperature 293K

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