1S7J
Crystal structure of phenazine biosynthesis protein PhzF family (Enterococcus faecalis)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-12-08 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.800, 85.740, 105.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.920 - 2.300 |
R-factor | 0.229 |
Rwork | 0.229 |
R-free | 0.27300 |
Structure solution method | MIR |
RMSD bond length | 0.011 |
RMSD bond angle | 1.500 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.073 | 0.176 |
Number of reflections | 25009 | |
<I/σ(I)> | 16.2 | 3.2 |
Completeness [%] | 99.1 | 93.9 |
Redundancy | 18 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 290 | 100 mM HEPES-Na, pH7.5, 27% PEG 3350, 200 mM MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 290K, pH 7.50 |