1S7H
Structural Genomics, 2.2A crystal structure of protein YKOF from Bacillus subtilis
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-03-10 |
Detector | SBC-2 |
Wavelength(s) | 0.9795, 0.9798 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 169.740, 55.078, 85.493 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.200 - 2.200 |
R-factor | 0.221 |
Rwork | 0.221 |
R-free | 0.28100 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.300 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.250 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.089 | 0.400 |
Number of reflections | 81572 | |
<I/σ(I)> | 23.4 | 3.85 |
Completeness [%] | 99.0 | 95.2 |
Redundancy | 9.2 | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.2M MgCl2 Hexahydrate, 0.1M Tris , 25% PEG 3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |