1S7F
RimL- Ribosomal L7/L12 alpha-N-protein acetyltransferase crystal form I (apo)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 77 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-12-13 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 68.400, 68.400, 89.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.24200 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 14015 | |
| <I/σ(I)> | 18.2 | |
| Completeness [%] | 92.7 | 83.5 |
| Redundancy | 3.2 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | vapor diffusion under oil | 7.8 | 293 | triethanolamine, ammonium sulfate, sodium malonate, pH 7.8, vapor diffusion under oil, temperature 293K |
