1S77
T7 RNAP product pyrophosphate elongation complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.05 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 127.738, 141.843, 142.856 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.690 |
| R-factor | 0.251 |
| Rwork | 0.251 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | T7 RNAP elongation post-translocated complex |
| RMSD bond length | 0.008 |
| RMSD bond angle | 20.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Number of reflections | 35620 | |
| Completeness [%] | 0.992 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 * | 21 * | T7RNAP, DNA,RNA, 3'deoxyATP, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris-HCl | 50 (mM) | pH7.0 |
| 2 | 1 | reservoir | PEG8000 | 8 (%) | |
| 3 | 1 | reservoir | 200 (mM) | ||
| 4 | 1 | reservoir | magnesium acetate | 10 (mM) | |
| 5 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 6 | 1 | drop | Tris-HCl | 50000 (nM) | pH7.5 |
| 7 | 1 | drop | 20000 (nM) | ||
| 8 | 1 | drop | magnesium acetate | 10000 (nM) |
