1S6V
Structure of a cytochrome c peroxidase-cytochrome c site specific cross-link
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-03-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.240, 107.920, 88.380 |
| Unit cell angles | 90.00, 104.42, 90.00 |
Refinement procedure
| Resolution | 45.650 - 1.880 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.910 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.054 | 0.573 |
| Number of reflections | 60567 | |
| <I/σ(I)> | 13.3 | 3 |
| Completeness [%] | 91.9 | 85.9 |
| Redundancy | 4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | PEG 3350, KI, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
