1S6M

Conjugative Relaxase Trwc In Complex With Orit DNA. Metal-Bound Structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	110
Detector technology	CCD
Collection date	2003-03-19
Detector	MARRESEARCH
Wavelength(s)	0.983994
Spacegroup name	P 61 2 2
Unit cell lengths	92.731, 92.731, 208.855
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.850 - 2.280
R-factor	0.206
Rwork	0.206
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1omh
RMSD bond length	0.013
RMSD bond angle	1.340
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	45.264
High resolution limit [Å]	2.280
Number of reflections	24598
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.6	296	25% PEGMM 2000, 0.2 M AMMONIUM SULPHATE, 0.1 M SODIUM ACETATE, PH 4.6, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 296K