1S6C

Crystal structure of the complex between KChIP1 and Kv4.2 N1-30

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-08-03
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0440
Spacegroup name	I 2 2 2
Unit cell lengths	74.744, 74.487, 85.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.790 - 2.000
Rwork	0.206
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g8i
RMSD bond length	0.006 *
RMSD bond angle	1.272 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.050 *
Total number of observations	148552 *
Number of reflections	16062
Completeness [%]	100.0 *	94.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	23 *	PEG 1000, magnesium nitrate, sodium cacodylate, DTT, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	drop	Tris	5 (mM)	pH8.0
3	1	drop		50 (mM)
4	1	reservoir	sodium cacodylate	100 (mM)	pH6.0
5	1	reservoir		100 (mM)
6	1	reservoir	PEG1000	20 (%)