1S6C
Crystal structure of the complex between KChIP1 and Kv4.2 N1-30
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-03 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0440 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 74.744, 74.487, 85.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.790 - 2.000 |
| Rwork | 0.206 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g8i |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.272 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 * | |
| Total number of observations | 148552 * | |
| Number of reflections | 16062 | |
| Completeness [%] | 100.0 * | 94.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 23 * | PEG 1000, magnesium nitrate, sodium cacodylate, DTT, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | drop | Tris | 5 (mM) | pH8.0 |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | reservoir | sodium cacodylate | 100 (mM) | pH6.0 |
| 5 | 1 | reservoir | 100 (mM) | ||
| 6 | 1 | reservoir | PEG1000 | 20 (%) |
