1S5V
Crystal Structure Analysis of a mutant of DIHYDRODIPICOLINATE SYNTHASE--residue Tyr107 to Phe107
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2002-10-20 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 120.888, 120.888, 110.202 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.940 - 2.350 |
R-factor | 0.19705 |
Rwork | 0.195 |
R-free | 0.23174 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dhp |
RMSD bond length | 0.011 |
RMSD bond angle | 1.313 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.410 |
High resolution limit [Å] | 2.350 | 2.350 |
Rmerge | 0.114 | 0.280 |
Number of reflections | 38813 | |
Completeness [%] | 98.6 | 95.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 10 | 277 | potassium phosphate (0.0012ml, 1.8M, pH10), N-octyl-beta-R-glucopyrandoside (0.0006ml, 6% w/v), VAPOR DIFFUSION, HANGING DROP, temperature 277K |