1S5U

Crystal Structure of Hypothetical Protein EC709 from Escherichia coli

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-12-18
Detector	SBC-2
Wavelength(s)	1.28, 0.9795, 0.9793, 0.956
Spacegroup name	P 1
Unit cell lengths	57.108, 70.187, 71.525
Unit cell angles	103.47, 95.15, 97.58

Refinement procedure

Resolution	23.640 - 1.700
R-factor	0.2
Rwork	0.200
R-free	0.23000
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.200
Data scaling software	HKL-2000
Phasing software	autoSHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.640	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.041	0.227
Number of reflections	102856
<I/σ(I)>	14.2	3.34
Completeness [%]	88.7	46.9
Redundancy	2.28	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	298	ammonium sulfate, sodium cacodylate, sodium chloride, ethylene glycol, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K