1S5U
Crystal Structure of Hypothetical Protein EC709 from Escherichia coli
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-18 |
| Detector | SBC-2 |
| Wavelength(s) | 1.28, 0.9795, 0.9793, 0.956 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.108, 70.187, 71.525 |
| Unit cell angles | 103.47, 95.15, 97.58 |
Refinement procedure
| Resolution | 23.640 - 1.700 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.23000 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data scaling software | HKL-2000 |
| Phasing software | autoSHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.640 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.041 | 0.227 |
| Number of reflections | 102856 | |
| <I/σ(I)> | 14.2 | 3.34 |
| Completeness [%] | 88.7 | 46.9 |
| Redundancy | 2.28 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 298 | ammonium sulfate, sodium cacodylate, sodium chloride, ethylene glycol, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
