1S5G
Structure of Scallop myosin S1 reveals a novel nucleotide conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-11-24 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 84.051, 50.935, 161.590 |
Unit cell angles | 90.00, 98.36, 90.00 |
Refinement procedure
Resolution | 43.440 - 3.100 |
R-factor | 0.233 |
Rwork | 0.233 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kk7 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.210 |
High resolution limit [Å] | 3.100 | 3.100 |
Number of reflections | 21309 | |
<I/σ(I)> | 20.6 | 4.2 |
Completeness [%] | 85.2 | 59.1 |
Redundancy | 4.2 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 300 | PEG 8000, magnesium chloride, ADP, Glycerol, MES, TMAO, cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 300K |