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1S52

Thr24Val Bacteriorhodopsin

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsALS BEAMLINE 8.2.2
Synchrotron siteALS
Beamline8.2.2
Temperature [K]100
Detector technologyCCD
Collection date2003-11-16
DetectorADSC QUANTUM 315
Wavelength(s)1.000
Spacegroup nameP 1 21 1
Unit cell lengths44.414, 108.554, 55.818
Unit cell angles90.00, 113.40, 90.00
Refinement procedure
Resolution30.000 - 2.300
R-factor0.19901
Rwork0.199
R-free0.26600

*

Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1py6
RMSD bond length0.012
RMSD bond angle1.400
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareCNS
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]30.0002.360
High resolution limit [Å]2.2802.280
Rmerge0.139
Number of reflections20650
<I/σ(I)>8.2
Completeness [%]90.391.8
Redundancy2.8
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion

*

3.737

*

sodium phosphate, hexanediol, pH 3.7, bicelle hanging drop vapor diffusion, temperature 310K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11reservoir2.2 (M)pH3.7
21reservoirhexanediol0.060 (M)
31dropprotein/bicelle solution40 (%(w/v))

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PDB entries from 2024-11-06

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