1S4U

Crystal Structure analysis of the beta-propeller protein Ski8p

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-10
Detector	MARRESEARCH
Wavelength(s)	0.9789, 0.9791, 0.9724
Spacegroup name	P 21 21 21
Unit cell lengths	65.555, 66.791, 81.459
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.960 - 2.100
R-factor	0.2529
Rwork	0.251
R-free	0.28332
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.060
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.154
High resolution limit [Å]	2.100	2.100
Number of reflections	21439
Completeness [%]	1.0	0.988

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	PEG4000, Sodium citrate, Ethylene glycol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K