1S4S

Reaction Intermediate in the Photocycle of PYP, intermediate occupied between 100 micro-seconds to 5 milli-seconds

Experimental procedure

Experimental method	LAUE
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	288
Detector technology	IMAGE PLATE
Collection date	2000-12-01
Detector	MARRESEARCH
Wavelength(s)	1.0-1.5
Spacegroup name	P 63
Unit cell lengths	66.900, 66.900, 40.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 1.900
R-factor	0.2446
Rwork	0.245
R-free	0.28920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2phy
Data reduction software	LaueView
Data scaling software	LaueView
Refinement software	XTALVIEW

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	1.900
Number of reflections	8326
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	Ammoniumsulphate, Potassium Phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	7	291	Ammoniumsulphate, Potassium Phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K