1S4N
Crystal structure of yeast alpha1,2-mannosyltransferase Kre2p/Mnt1p
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0098,1.0077,0.9840,1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.694, 101.068, 62.036 |
| Unit cell angles | 90.00, 98.81, 90.00 |
Refinement procedure
| Resolution | 19.960 - 2.010 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.23200 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.010 | 2.000 |
| Rmerge | 0.050 | 0.192 |
| Number of reflections | 49965 | |
| <I/σ(I)> | 58.4 | 16.4 |
| Completeness [%] | 97.0 | 88.3 |
| Redundancy | 22.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 293 | PEG 2000 MME, HEPES, SODIUM CHLORIDE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.50 |
