1S4M
Crystal structure of flavin binding to FAD synthetase from Thermotoga maritina
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-10-07 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.093, 82.163, 66.754 |
Unit cell angles | 90.00, 116.57, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.100 |
R-factor | 0.223 |
Rwork | 0.212 |
R-free | 0.25870 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.672 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 40525 | |
<I/σ(I)> | 17.8 | |
Completeness [%] | 94.0 | 82.7 |
Redundancy | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 300 | sodium citrate, PEG 4000, glycerol, propanol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 300K |