1S4K
Putative cytoplasmic protein from Salmonella typhimurium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 150 |
Detector technology | CCD |
Collection date | 2003-12-21 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.97885 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 85.596, 90.258, 78.669 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.890 - 1.900 |
R-factor | 0.191 |
Rwork | 0.191 |
R-free | 0.21900 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.000 |
Data reduction software | d*TREK |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 22681 | |
<I/σ(I)> | 3.34 | |
Completeness [%] | 86.0 | 59.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 294 | NaH2PO4, K2HPO4, acetate, sucrose, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |