1S44
The structure and refinement of apocrustacyanin C2 to 1.6A resolution and the search for differences between this protein and the homologous apoproteins A1 and C1.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS |
Synchrotron site | SRS |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2002-06-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.167, 79.879, 109.562 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 64.550 - 1.600 |
R-factor | 0.20514 |
Rwork | 0.203 |
R-free | 0.24744 |
Structure solution method | not applicable |
Starting model (for MR) | 1h91 |
RMSD bond length | 0.026 |
RMSD bond angle | 2.265 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.970 | 1.680 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.132 | 0.662 |
Number of reflections | 47856 | |
<I/σ(I)> | 4.7 | 1.1 |
Completeness [%] | 98.6 | 97.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 9 | 298 | 2.3M ammonium sulphate, 20% glycerol, pH 9, VAPOR DIFFUSION, temperature 298K |