1S3Q

Crystal structures of a novel open pore ferritin from the hyperthermophilic Archaeon Archaeoglobus fulgidus

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-03-27
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9720, 0.9800, 0.9797
Spacegroup name	C 2 2 21
Unit cell lengths	183.437, 187.831, 178.089
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.010 - 2.100
R-factor	0.18055
Rwork	0.179
R-free	0.21771
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.571
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.069
Number of reflections	174236
<I/σ(I)>	13.32	5.16
Completeness [%]	97.7	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	microbatch under oil	7.5	293	PEG 400, HEPES, MgCl2, pH 7.5, microbatch under oil, temperature 293K