1S3L
Structural and Functional Characterization of a Novel Archaeal Phosphodiesterase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-11-21 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9790 |
Spacegroup name | I 41 |
Unit cell lengths | 83.722, 83.722, 149.472 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.410 - 2.400 |
R-factor | 0.226 |
Rwork | 0.226 |
R-free | 0.25300 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.900 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 20082 | |
<I/σ(I)> | 24.5 | 5.5 |
Redundancy | 12 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.7M AMMONIUM SULFATE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.7M AMMONIUM SULFATE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.7M AMMONIUM SULFATE, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |