1S3J
X-ray crystal structure of YusO protein from Bacillus subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-11-25 |
Detector | SBC-2 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.029, 71.912, 96.642 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.710 - 2.250 |
R-factor | 0.221 |
Rwork | 0.219 |
R-free | 0.27300 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.222 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.300 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.101 | 0.510 |
Number of reflections | 16195 | |
<I/σ(I)> | 8 | |
Completeness [%] | 99.5 | 94.5 |
Redundancy | 13.59 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 294 | Ammonium Sulfate, Bis-Tris, Pentaerythritol Ethoxylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K, pH 6.50 |