1S3F
Purine 2'-deoxyribosyltransferase + selenoinosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 8-BM |
Synchrotron site | APS |
Beamline | 8-BM |
Temperature [K] | 170 |
Detector technology | CCD |
Collection date | 2002-01-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9791 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.582, 79.582, 185.727 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.940 - 2.200 |
Rwork | 0.274 |
R-free | 0.29300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.066 | 0.256 |
Number of reflections | 35106 | |
<I/σ(I)> | 6.7 | 2.8 |
Completeness [%] | 98.1 | 100 |
Redundancy | 13 | 12.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 2.0 M ammonium sulfate, 100mM tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |