1S36
Crystal structure of a Ca2+-discharged photoprotein: Implications for the mechanisms of the calcium trigger and the bioluminescence
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-20 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| 格子定数 [Å] | 53.445, 53.445, 144.032 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 50.000 - 1.960 |
| R因子 | 0.22459 |
| Rwork | 0.223 |
| R-free | 0.25922 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jf2 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.012 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.207 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | LSCALE |
| Refinement software | REFMAC (5.1.24) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 72.460 | 2.050 |
| 分解能 [Å] (高) | 1.960 | 1.960 |
| 独立反射数 | 15775 | |
| <I/σ(I)> | 9.37 | 2.11 |
| 完全性 [%] | 1.0 | 0.97 |
| 冗長性 | 14.91 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | modified microbatch | 7.5 | 277 | 1.5 M tri-sodium citrate, 0.1 M Na-HEPES, pH 7.5, modified microbatch, temperature 277K |
