1S35

Crystal Structure of Repeats 8 and 9 of Human Erythroid Spectrin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 5ID-B
Synchrotron site	APS
Beamline	5ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2003-02-02
Detector	MARRESEARCH
Wavelength(s)	0.9796,0.9794,0.9642
Spacegroup name	P 41 21 2
Unit cell lengths	122.151, 122.151, 49.548
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.920 - 2.400
Rwork	0.222
R-free	0.25900
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.000
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.920	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.088	0.303
Number of reflections	15165
<I/σ(I)>	7.6	2.7
Completeness [%]	99.6	99.8
Redundancy	6.3	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	0.1 M Sodium Citrate, 0.3 M Ammonium Sulfate, 1.2-1.3 M Lithium Sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K