1S31
Crystal Structure Analysis of the human Tub protein (isoform a) spanning residues 289 through 561
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2003-08-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9814 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 149.981, 149.981, 149.981 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.474 - 2.704 |
| R-factor | 0.1836 |
| Rwork | 0.181 |
| R-free | 0.22640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c8z |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE (CCP4 4.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.096 | 0.344 |
| Number of reflections | 15503 | |
| <I/σ(I)> | 30.9 | 10.1 |
| Completeness [%] | 100.0 | 99.7 |
| Redundancy | 24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277.2 | 16% PEG 400, 5mM Magnesium Chloride, 100mM Sodium Acetate, 15mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 277.2K, pH 5.0 |
