1S2P
The structure and refinement of apocrustacyanin C2 to 1.3A resolution and the search for differences between this protein and the homologous apoproteins A1 and C1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS |
| Synchrotron site | SRS |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.148, 79.846, 110.263 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.200 - 1.300 |
| R-factor | 0.19044 |
| Rwork | 0.189 |
| R-free | 0.21473 |
| Structure solution method | not applicable |
| Starting model (for MR) | 1h91 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.573 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.200 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.080 | 0.769 |
| Number of reflections | 87664 | |
| <I/σ(I)> | 5.4 | 0.8 |
| Completeness [%] | 97.2 | 89.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | 2.3M ammonium sulphate, 5%MPD, 0.1M Tris HCl, pH 7, VAPOR DIFFUSION, temperature 298K |
