1S2O

X-Ray structure of the sucrose-phosphatase (SPP) from Synechocystis sp. PCC6803 at 1.40 A resolution

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-11
Detector	MARRESEARCH
Wavelength(s)	1.483148
Spacegroup name	P 1 21 1
Unit cell lengths	46.070, 51.760, 52.140
Unit cell angles	90.00, 101.70, 90.00

Refinement procedure

Resolution	20.000 - 1.400
Rwork	0.182
R-free	0.20000
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	1.312
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.400
Rmerge	0.057
Number of reflections	45838
<I/σ(I)>	12.58
Completeness [%]	96.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	NaFormate, NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP		293	NaFormate, NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP		293	NaFormate, NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K