1S2N
Crystal structure of a cold adapted subtilisin-like serine proteinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-09-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.425, 36.842, 143.065 |
| Unit cell angles | 90.00, 93.88, 90.00 |
Refinement procedure
| Resolution | 29.110 - 2.440 |
| R-factor | 0.18385 |
| Rwork | 0.177 |
| R-free | 0.24744 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Homology model based on PDB entry 1IC6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.065 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.530 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Number of reflections | 17252 | |
| <I/σ(I)> | 10.4 | 3.4 |
| Completeness [%] | 97.6 | 92.1 |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | PEG 4000, isopropanol, HEPES, pH 8.0, VAPOR DIFFUSION, temperature 293K |
