1S2M
Crystal Structure of the DEAD box protein Dhh1p
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9195, 0.9198, 0.9134 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.210, 80.410, 54.824 |
| Unit cell angles | 90.00, 100.57, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.20216 |
| Rwork | 0.200 |
| R-free | 0.23356 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.085 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.220 |
| High resolution limit [Å] | 2.100 | 2.120 |
| Number of reflections | 23923 | |
| Completeness [%] | 99.5 | 99.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | MES, Potassium bromide, PEG400, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
