1S2K
Structure of SCP-B a member of the Eqolisin family of Peptidases in a complex with a Tripeptide Ala-Ile-His
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-09-13 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 109.369, 109.369, 113.829 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.000 - 2.000 |
| Rwork | 0.234 |
| R-free | 0.25600 |
| Structure solution method | MIR |
| Starting model (for MR) | 1s2b |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.050 |
| Number of reflections | 27676 |
| <I/σ(I)> | 19.2 |
| Completeness [%] | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 295 | 42% sat'd ammonium sulfate 0.1M sodium acetate, 10% v/v ethylene glycol, 10 mM Angiotensin II, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
