1S2D
Purine 2'-Deoxyribosyl complex with arabinoside: Ribosylated Intermediate (AraA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 170 |
| Detector technology | CCD |
| Collection date | 2002-01-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.587, 79.587, 184.973 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.740 - 2.100 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.25300 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.058 | 0.127 |
| Number of reflections | 35292 | |
| <I/σ(I)> | 22 | 10.1 |
| Completeness [%] | 98.7 | 98.6 |
| Redundancy | 8.3 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 2.0 M ammonium sulfate, 100mM Tris , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
