1S28
Crystal Structure of AvrPphF ORF1, the Chaperone for the Type III Effector AvrPphF ORF2 from P. syringae
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97925, 0.97936, 0.97167 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.053, 94.920, 104.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.970 - 3.000 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.29000 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.900 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.096 | 0.257 |
| Number of reflections | 18973 | |
| <I/σ(I)> | 26.9 | 7.66 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.4 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | Ammonium Sulfate,Tris 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
