1S21
Crystal Structure of AvrPphF ORF2, A Type III Effector from P. syringae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-08-28 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.689, 68.334, 88.365 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.630 - 2.000 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.24100 |
Structure solution method | MIRAS |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.054 | 0.233 |
Number of reflections | 16442 | |
<I/σ(I)> | 18.9 | 6.1 |
Completeness [%] | 99.8 | 99.1 |
Redundancy | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | PEG8000, Bis-Tris, Magnesium Acetate and glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | PEG8000, Bis-Tris, Magnesium Acetate and glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |