1S1M

Crystal Structure of E. Coli CTP Synthetase

Experimental procedure

Experimental method	THREE-WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-05-10
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	165.507, 106.807, 130.033
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.214
Rwork	0.214
R-free	0.28100
Structure solution method	HG-MAD
RMSD bond length	0.009
RMSD bond angle	1.900
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELXS
Refinement software	TNT (version 5f)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.370
High resolution limit [Å]	2.300	2.300
Number of reflections	101701
Completeness [%]	99.0	98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5	275	ammonium sulfate, sodium iodide, tris, tcep, magnesium chloride, utp, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 275K, pH 8.50
1		8.5	275	ammonium sulfate, sodium iodide, tris, tcep, magnesium chloride, utp, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 275K, pH 8.50
1		8.5	275	ammonium sulfate, sodium iodide, tris, tcep, magnesium chloride, utp, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 275K, pH 8.50