1S1K
INFLUENCE OF GROOVE INTERACTIONS ON DNA HOLLIDAY JUNCTION FORMATION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2002-01-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 32.957, 32.957, 88.556 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 17.490 - 1.900 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p4z |
| RMSD bond length | 0.067 |
| RMSD bond angle | 6.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.500 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.056 | 0.245 |
| Number of reflections | 2528 | |
| <I/σ(I)> | 4.98 | |
| Completeness [%] | 96.3 | 72.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 5mM TRIS(HCl), 23mM CaAcetate, 16% MPD, .6mM DNA against 28% MPD at RT, pH 7.50, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | TRIS(HCl) | ||
| 2 | 1 | 1 | CaAcetate | ||
| 3 | 1 | 1 | 16% MPD | ||
| 4 | 1 | 1 | DNA | ||
| 5 | 1 | water | |||
| 6 | 1 | 28% MPD | |||
| 7 | 1 | TRIS(HCl) |
