1S1G
Crystal Structure of Kv4.3 T1 Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 143 |
| Detector technology | CCD |
| Collection date | 2001-05-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 84.230, 84.230, 104.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.600 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB CODE 3kvt |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 12263 | |
| Completeness [%] | 96.1 | 87 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 18 * | Sodium Phosphate/Potassium Phosphate, Lisium Sulfate, CAPS, pH 10.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6-7 (mg/ml) | |
| 2 | 1 | drop | Tris | 25 (mM) | pH7.4 |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 10 (mM) | |
| 5 | 1 | drop | 1 (mM) | ||
| 6 | 1 | reservoir | potassium sodium phosphate | 2.0 (M) | |
| 7 | 1 | reservoir | 0.2 (M) | ||
| 8 | 1 | reservoir | CAPS | 0.1 (M) | pH10.5 |
