1S1F
Crystal Structure of Streptomyces Coelicolor A3(2) CYP158A2 from antibiotic biosynthetic pathways
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2002-10-26 |
Detector | BRUKER PROTEUM 300 |
Wavelength(s) | 0.9975,1.0055,1.0090 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.250, 65.553, 104.245 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.260 - 1.500 |
R-factor | 0.203 |
Rwork | 0.202 |
R-free | 0.21600 |
Structure solution method | combination with MAD phase and SIR phase |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (V. 2.03) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.260 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.067 | 0.630 |
Number of reflections | 55989 | |
<I/σ(I)> | 27.6 | 1.5 |
Completeness [%] | 92.5 | 85.5 |
Redundancy | 25.8 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | sodium, malonic acid, glycerol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | sodium, malonic acid, glycerol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | sodium, malonic acid, glycerol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | sodium, malonic acid, glycerol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |